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[1]李青,王博,邱弘毅,等.昼夜节律紊乱对肠道微生态和粪便代谢组学影响的实验研究[J].国际消化病杂志,2022,02:126-133.
　LI Qing,WANG Bo,QIU Hongyi,et al.Experimental study on the influence of circadian rhythm disturbance on intestinal microecology and fecal metabolites[J].International Journal of Digestive Disease,2022,02:126-133.
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昼夜节律紊乱对肠道微生态和粪便代谢组学影响的实验研究(PDF)

《国际消化病杂志》[ISSN:1673-534X/CN:31-1953/R]

期数:
2022年02期
页码:
126-133
栏目:
论著
出版日期:
2022-04-20

文章信息/Info

Title:
Experimental study on the influence of circadian rhythm disturbance on intestinal microecology and fecal metabolites
作者:
李青; 王博; 邱弘毅; 颜秀娟; 程莉; 王倩倩; 陈胜良
200001 上海交通大学医学院附属仁济医院消化科
Author(s):
LI Qing;  WANG Bo;  QIU Hongyi;  YAN Xiujuan;  CHENG Li; WANG Qianqian;  CHEN Shengliang.
Department of Gastroenterology and Hepatology, Renji Hospital,School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, China
关键词:
昼夜节律; 肠道微生态; 代谢物; 消化; 吸收; 脑- 肠轴
Keywords:
Circadian rhythm;  Intestinal microecology;  Metabolites;  Digestion;  Absorption;  Braingutaxis
分类号:
-
DOI:
10.3969/j.issn.1673-534X.2022.02.012
文献标识码:
-
摘要:
目的 探究昼夜节律紊乱对肠道微生态和粪便代谢物特征的影响。 方法 选取12 只健康C57BL/6J 小鼠（雄性，6～8 周龄，18～25 g）作为研究对 象，随机分为2 组，每组6 只。采用转换饲养环境光照节律的方法模拟昼夜节律 变化，建立昼夜节律紊乱小鼠模型。4 个月后，收集小鼠粪便和空肠内容物，采用 16S rRNA 测序法分析小鼠空肠和结肠内菌群的变化，采用靶向代谢组学分析粪便 样品中代谢组学的变化。结果 与昼夜节律正常组（Con 组，n＝6）比较，昼夜节 律紊乱组（JL 组，n＝5）小鼠结肠菌群的丰度和多样性均降低，其中Bacteroidota 门细菌丰度显著降低，Firmicutes 门和 Actinobacteria 门细菌丰度均增高。粪便代谢 组学结果显示，与Con 组比较，JL 组小鼠粪便中氨基酸水平显著降低（14.11% 比 30.04%，P＜0.01）；两组小鼠粪便中碳水化合物和短链脂肪酸水平的差异均无统计 学意义（P 均＞0.05）。结论 昼夜节律改变能够引起小鼠肠道微生态及粪便代谢物 的变化。
Abstract:
Objective This study aims to explore the influence of circadian rhythm disorder on intestinal microecology changes and fecal metabolites characteristics. Methods Twelve healthy C57BL/6J mice (male, 6 to 8 weeks old, 18 to 25 g) were selected and randomly assigned to two groups with 6 mice in each group. The change of circadian rhythm was simulated by changing the light rhythm of the rearing environment, and the mouse model of circadian rhythm disorder was established. After 4 months, the stool samples and jejunal contents were collected. The jejunal contents and microbiota of fecal samples were analyzed by 16S ribosomal RNA sequencing. Targeted metabolomics was used to explore the differences of fecal metabolites between the two groups. Results Compared with the normal circadian rhythm group (Con group, n＝6), the abundance and diversity of colonic flora of the mice in the circadian rhythm disorder group (JL group, n＝5) are decreased, and the abundance of Bacteroidota is significantly decreased. The bacterial abundance of Firmicutes and Actinobacteria increased. Fecal metabolomics results show that compared with the Con group, the levels of amino acids in the feces of the mice in the JL group are significantly lower (14.11% versus 30.04%, P＜0.01). There is no difference in the levels of carbohydrates and short-chain fatty acids in the feces of the two groups of mice. Conclusion Disturbances in the circadian rhythm can indeed trigger changes in the intestinal microecology and fecal metabolites in mice.

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备注/Memo

备注/Memo:
通信作者：陈胜良，Email: slchenmd@homail.com
更新日期/Last Update: 2022-04-20