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《国际消化病杂志》[ISSN:1673-534X/CN:31-1953/R]

期数:

2023年06期

页码:

388-393

栏目:

论著

出版日期:

2023-12-30

文章信息/Info

Title:

Effect of SDC-1 regulating TGF-β1/THBS1 signaling pathway on progress of liver fibrosis

作者:

羊 丹; 田婉婷; 徐 菁

723000 陕西汉中，三二〇一医院消化内科

Author(s):

YANG Dan;  TIAN Wanting;  XU Jing.

Department of Gastroenterology, 3201 Hospital, Hanzhong 723000, China

关键词:

慢性乙型肝炎; 肝纤维化; 多配体蛋白聚糖-1; 血小板反应蛋白1; 肝星状细胞

Keywords:

Chronic hepatitis B;  Liver fibrosis;  Syndecan-1;  Thrombospondin-1;  Hepatic stellate cell

分类号:

-

DOI:

10.3969/j.issn.1673-534X.2023.06.007

文献标识码:

-

摘要:

目的探究多配体蛋白聚糖-1（SDC-1）在肝纤维化进程中的作用机制。方法选择2021年4月至2023年4月于三二〇一医院住院治疗并行肝穿刺活体组织病理检查的102例慢性乙型肝炎患者作为研究对象。采集患者的空腹静脉血，采用ELISA法检测患者血清SDC-1的表达水平，并比较不同肝纤维化分期患者血清SDC-1表达水平的差异。观察经转化生长因子-β1（TGF-β1）诱导活化的LX-2细胞中SDC-1表达水平的变化，以及敲低SDC-1表达对活化的LX-2细胞中α-平滑肌肌动蛋白（α-SMA）、血小板反应蛋白1（THBS1）表达水平及细胞增殖、细胞周期的影响。结果随着肝纤维化程度逐渐加重，慢性乙型肝炎患者血清SDC-1的表达水平呈升高趋势（P＜0.05）。与阴性对照（NC）组相比，TGF-β1组α-SMA、SDC-1蛋白及其mRNA的相对表达量均显著升高，细胞增殖活性显著增强，G1期细胞占比显著降低，S期和G2期细胞占比显著升高（P均＜0.05）。与NC小干扰RNA（siRNA）组相比，SDC-1siRNA组α-SMA、TGF-β1、THBS1蛋白及其mRNA的相对表达量均显著降低，细胞增殖活性显著减弱，G1期细胞占比显著升高，S期和G2期细胞占比显著降低（P均＜0.05）。结论敲低SDC-1表达可能通过调控TGF-β1/THBS1信号通路，抑制肝星状细胞（HSC）的活化及增殖，阻滞HSC由G1期进入S期和G2期，进而发挥抑制肝纤维化进展的作用。

Abstract:

Objective? This paper intends to explore the role of syndecan-1 (SDC-1) in the progression of liver fibrosis. Methods? From April 2021 to April 2023, a total of 102 patients with chronic hepatitis B who were hospitalized and underwent liver biopsy in the 3201 Hospital were selected as the research objects. The fasting venous blood was collected and the expression of serum SDC-1 was measured using the ELISA method. The expression of serum SDC-1 was compared among patients with different stages of liver fibrosis. The changes of SDC-1 expression in activated LX-2 cells induced by transforming growth factor-β1 (TGF-β1), and the effects of knockdown of SDC-1 on the expression of α-smooth muscle actin (α-SMA), thrombospondin 1(THBS1), cell proliferation and cell cycle in activated LX-2 cells were observed. Results? With the aggravation of liver fibrosis stages, the expression level of serum SDC-1 in patients with chronic hepatitis B increases (P＜0.05). Compared with the negative control (NC) group, the relative expressions of α-SMA, SDC-1 protein and their mRNA in the TGF-β1 group are increased, the cell proliferation activity is increased, the proportion of G1 phase cells is decreased, and the proportion of S phase and G2 phase cells are increased, with statistically significant differences (P＜0.05). Compared with the NC siRNA group, the relative expressions of α-SMA, TGF-β1, THBS1 protein, and their mRNA in the SDC-1 siRNA group are decreased, the cell proliferation activity is decreased, the proportion of G1 phase cells is increased, and the proportion of S phase and G2 phase cells are decreased, with statistically significant differences (P＜0.05). Conclusion? Knockdown of SDC-1 expression may inhibit the activation and proliferation of hepatic stellate cells by regulating TGF-β1/THBS1 signaling pathway, and block the progression of hepatic stellate cells from entering the G1 phase to the S phase and the G2 phase, and then play a role in inhibiting the process of liver fibrosis.

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备注/Memo

备注/Memo:

通信作者：徐菁，Email: 1600117355@qq.com

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更新日期/Last Update: 1900-01-01